Using 83 female moths of the Kangda×9906 strain, we investigated oviposition quantity, egg weight, and disease resistance during a two-day oviposition period.The results revealed that both egg quantity and average weight per egg during 0~24 h were highly significantly higher than during 24~48 h.Maximal egg weight difference(1.44 mg/egg)occurred when>90% of total eggs were laid within 0~24 h.Intra-period egg weight variation was far smaller than inter-period quantity variation.In moth groups with≥80% eggs laid in 0~24 h,ApNPV(Antheraea pernyi Nucleopolyhedrovirus)was applied via surface contamination.LC50 for 0~24 h eggs(9.67×106)was 15-fold higher than for 24~48 h eggs(5.74×105).0~24h eggs showed significantly stronger resistance to identical virus concentrations.These results support prioritizing 0~24h eggs for selective breeding and preservation in spring tussah silkworm propagation.