Abstract:To implement the big food concept and establish a diversified food supply system while promoting the transformation of tussah resources from regional ingredients to standardized commodities, this study innovatively developed a combined technology of modified atmosphere preservation and counter-pressure high-temperature sterilization for tender tussah pupae (immortal pupa), a characteristic resource in Northeast China. Through the L9(34) orthogonal test, process parameters were optimized for both room temperature (25℃) and low-temperature (4℃) storage. Key results demonstrate that optimal room temperature conditions—100% CO2 atmosphere, 60-minute sterilization, and triple-layer high-barrier packaging—achieved a 3-month shelf life; meanwhile, low-temperature storage with 80% CO2+20% N2,50-minute sterilization,and triple-layer packaging extended shelf life to 6 months.The proposed technique effectively preserved pupal morphology,color,flavor,and nutritional value,resolving texture deterioration issues (e.g., roughness and quality loss) associated with conventional freezing methods.